Coding

Part:BBa_K3796208:Design

Designed by: Yang Feiyue   Group: iGEM21_CAU_China   (2021-09-24)


LacIq (coding for lac repressor)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

LacIq is usually coupled with certain inducible promoters and in our experiments we used tac promoter carried by the plasmid pXMJ19.


Source

You can find this in many bacteria genome and we amplified this from the plasmid pXMJ19.

References